Hygromycin phosphotransferase gene
WebHPT, the hygromycin phosphotransferase gene. (B) Target sites for eight sgRNAs targeting HD1. The gene structure (upper) and partial abbreviate sequence (lower) of HD1 were shown. The homeodomain and sgRNAs were represented as a black box and arrows, respectively. PAM sites (5′-NGG-3′) were indicated red at the end of each spacer sequence. WebHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. View Price and Availability Properties biological source Streptomyces hygroscopicus Quality Level
Hygromycin phosphotransferase gene
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Web5 mrt. 2024 · This gene cluster also contained a putative transcription factor gene (g432) and a self-resistance gene (g431). Hygromycin B may be used for activation of … Webselect transformants expressing the APT gene, which encodes an aminoglycoside phosphotransferase. Hygromycin B (HygB, Wako Pure Chemical Industries, Osaka, Japan) was added to YPD to a final concentration of 600mg/ml to select for transformants carrying the HPT gene, encoding HygB phosphotransferase.
WebDr. To currently works at the Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan. Dr. To published over 30 well-known research articles and reviews, 3 book chapters, and also received 2 patents. In addition, Dr. To has been serving as a reviewer in a range of journals. Since 2008, Dr. To has been operating and developing the Plant … WebThus, it directly affects the tea industry qualitatively and quantitatively. Solanum tuberosum class I chitinase gene (AF153195) is a plant pathogenesis-related gene. It was introduced into tea genome via Agrobacterium-mediated transformation with hygromycin phosphotransferase (hpt) gene conferring hygromycin resistance as plant selectable …
Web1 dec. 1991 · Abstract Abstract A general method of gene transfer that does not leave behind a selectable marker in the host genome is described. A luciferase gene was introduced into the tobacco genome by using the hygromycin phosphotransferase gene (hpt) as a linked selectable marker. Web1Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada Accepted on February 24, 2024 The neomycin phosphotransferase II (nptII) and hygromycin phosphotransferase II (hptII) genes, originally isolated from E. coli [1,2] are widely used as selectable markers in transgenic research [3-5].
WebComparative analyses of secondary metabolite biosynthetic gene clusters, ... (25 μg ml-1), and hygromycin (100 μg ml-1) as appropriate. XL1-blue was used for general cloning, ET12567 (pUZ8002) ... hygromycin phosphotransferase encoded by the vector is efficiently expressed and inactivates antibiotic early during the growth, ...
Web3 jan. 2024 · To create the ku70 gene deletion construct, 1.9 kb upstream and downstream of the ku70 gene and 0.55 kb of the Y. lipolytica tef1 promoter and the bacterial hygromycin phosphotransferase gene (hph) were amplified by PCR with oligo pairs (Additional file 3, Oligos) 1313/1314 (ku70 upstream), 1315/1321 (hph), 1322/1318 … r. kelly trial updateWebSontikun, Y., Chanprame, S., Srinives, P.... Proceedings of the 5th AG-BIO/PERDO graduate conference on agricultural biotechnology and KU-UT joint seminar II. Nakhon Pathom (Thailand). 2012. p. 45 (95 p.) sms diabetic suppliesWebGRITZ, L, PLASMID-ENCODED HYGROMYCIN-B RESISTANCE - THE SEQUENCE OF HYGROMYCIN-B PHOSPHOTRANSFERASE GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND SACCHAROMYCES-CEREVISIAE, GENE 25: 179 (1983). Google Scholar HORSCH, R.B., A SIMPLE AND GENERAL-METHOD FOR … r kelly \\u0026 mr biggs live video continuousWebThis enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups ( phosphotransferases) with an alcohol group as … r kelly truck partsWeb18 nov. 2024 · The hygromycin phosphotransferase (aphIV) antibiotic resistance marker gene was also used as target sequence for the fluorescent-based detection assay. Melting curve analysis was performed to ensure accuracy and … r kelly trial sentenceWebThe plasmid contains the hph gene from Klebsiella pneumoniae encoding hygromycin B phosphotransferase and confers resistance to the antibiotic hygromycin B of … r kelly turn back the hand a timeWeb18 mrt. 2016 · Gene: Hygromycin selectable marker from Escherichia coli – hygromycin phosphotransferase gene to confer. hygromycin resistance as a selectable marker . Gene: glucuronidase (GUS) reporter gene from Escherichia coli – reporter gene. 3`UTR: nos3′ UTR from Agrobacterium tumefaciens – terminator at end of GUS gene . smsd library catalog