Tf-centered y1h

Author: hzkn

August undefined, 2024

WebThe interaction between a protein and DNA is involved in almost all cellular functions, and is vitally important in cellular processes. Two complementary approaches are used to detect … WebGateway-compatible yeast one-hybrid (Y1H) assays present a convenient method to identify and characterize the repertoire of transcription factors that can bind a DNA sequence of interest.

Transcription Factor-Centered Yeast One-Hybrid Assay

WebThe preparation of expressional cDNA libraries for use in the hefen two-hybrid system will speed and efficient as usage the dedicated Clontech™ product, the MATCHMAKER Library Construction and Screening Kit 3. Which kit employs SMART engine for the rated of full-length cDNAs, in combination with cloning using homologous rekombination. Sadly, such … WebHere, we provide a TF-centered method based on the Y1H system to identify the motifs recognized by a defined TF, termed TF-centered Y1H. In this system, a random short DNA … fish in hawaii

Chapter 11

Web17 Aug 2024 · In this study, we employed a Transcription Factor-Centered Yeast one Hybrid (TF-Centered Y1H) screen to study the motifs recognized by ThWRKY4. In addition to the … Web17 Aug 2024 · In this study, we employed a Transcription Factor-Centered Yeast one Hybrid (TF-Centered Y1H) screen to study the motifs recognized by ThWRKY4. In addition to the W-box core cis-element (termed W-box), we identified that ThWRKY4 could bind to two other motifs: the RAV1A element (CAACA) and a novel motif with sequence of GTCTA (W-box … Web10 Aug 2014 · Taken together, these results demonstrated that TF-centered Y1H could identify quickly the motifs bound by a defined TF, representing a reliable and efficient … fish in hawaiian waters

Isolation of plant transcription factors using a modified yeast one ...

Yeast one-hybrid assays for gene-centered human gene ... - Nature

Web29 Jun 2024 · Using TF-centered Y1H and EMSA assays, we found that PsnICE1 can bind not only to E-box but also to H-box, ABRE and a newly discovered cis-acting element (IBS1) . These results indicated that PsnICE1 could bind to different cis-acting elements. The H-box is a cis-acting element that was first identified in the promoter of the chalcone synthase ... WebThe TF-centered Y1H method was used to identify the binding cis-elements of BplERF1. First, BplERF1 was constructed into the pGADT7-Rec2 vector using homologous … fish in handWeb11 Feb 2024 · In an effort to improve the assay we recently developed a Y1H system that uses a cell surface Gaussia luciferase reporter (gLUC59). Compared to other available … can chemotherapy affect teeth

"WebAbstract: Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms.The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to … " - Tf-centered y1h

Tf-centered y1h

What does biologically meaningful mean? A perspective on gene ...

Web10 Aug 2014 · It is demonstrated that TF-centered Y1H could identify quickly the motifs bound by a defined TF, representing a reliable and efficient approach with the advantages … WebIn the present study, we used a TF-centered yeast one-hybrid technology (TF-centered Y1H) to identify the cis- ... Here, we used the TF-centered Y1H to investigate the interaction of …

Did you know?

Web24 Jul 2014 · Here we present a computational method, called iRegulon, to identify master regulators and direct target genes in a human gene signature, i.e. a set of co-expressed genes. iRegulon relies on the analysis of the regulatory sequences around each gene in the gene set to detect enriched TF motifs or ChIP-seq peaks, using databases of nearly … WebTF-centered Y1H could identify quickly the motifs bound by a defined TF, representing a reliable and efficient approach with the advantages of Y1H. Therefore, this TF-centered Y1H may have a wide application in protein-DNA interaction studies. PMID: 29855957 DOI full text PubMed Download Citation (.nbib) Reference Type Journal Article

Web32.TF-centered Y1H系统的建立及柽柳NAC基因的抗逆功能研究. 33.红松无性系种子形态及营养成分变异研究. 34.杉木天冬酰胺合成酶基因的克隆与表达分析. 35.~(60)Co-γ辐射对多 … WebHere, we provide a TF-centered method based on the Y1H system to identify the motifs recognized by a defined TF, termed TF-centered Y1H. In this system, a random short DNA …

WebThe TF-centered Y1H method was used to identify the binding cis-elements of BplERF1. First, BplERF1 was constructed into the pGADT7-Rec2 vector using homologous recombination, the pGADT7-Rec2-BplERF1 plasmid and the pHIS2 element library plasmid were then co-transferred into Y187 yeast using yeast transformation kit (the Yeastmaker … Web15 Nov 2024 · TF-centered Y1H assay was used to find potential binding sites to PcbZIP44 in the PcPTS promoter. Furthermore, transient overexpression experiments and virus-induced gene silencing (VIGS) experiments were executed in this study to probe the role of PcbZIP44 in patchoulol biosynthesis.

Web7 Jun 2024 · The TF-centered Y1H (protein-to-DNA) method based on the Y1H system (Ji et al. 2014a) allowed us to screen for new motifs bound by BplMYB46. This system does not …

Web18 Jul 2024 · The Y1H is a powerful method for detecting DNA–protein interactions, and based on its original system (DNA-centered), multiple modifications have been developed to broaden its applications [11,12,13]. For example, a TF-centered Y1H has recently been developed by Ji et al., which could be used to identify the DNA motifs bound by a defined … can chemo save your lifeWeb1 Jan 2024 · TF-centered Y1H could identify quickly the motifs bound by a defined TF, representing a reliable and efficient approach with the advantages of Y1H. Therefore, this TF-centered Y1H may have a wide application in protein-DNA interaction studies. Full text links Read article at publisher's site (DOI): 10.1007/978-1-4939-7871-7_11 Citations & … fish in hawaiianWeb21 Oct 2024 · The TF-Centered Y1H system is a simple and efficient method for identification of the DNA sequence bound by transcription factors of interest [ 23, 24 ]. To identify the DNA sequences recognized by ANAC074, the random DNA insertion prey library was screened with ANAC074 (AT4G28530) cloned into pGADT7-Rec2 as bait using the … can chemo reduce the size of a tumorWebThe yeast one-hybrid (Y1H) is a powerful and widely used gene-centered system to identify DNA–protein interactions. However, a powerful and simple TF-centered method to study … can chemotherapy affect the thyroidWeb1 Oct 2024 · In this study, we employed a Transcription Factor-Centered Yeast one Hybrid (TF-Centered Y1H) screen to study the motifs recognized by ANAC074. In addition to the NACRS core cis-element, we identified that ANAC074 could bind to MybSt1, NRS1, and NRS2. Y1H and GUS assays showed that ANAC074 could bind the promoters of ethylene … fish in hawaii to eatWebWhen studying the genome-binding sites and functions of specific TF, a TF-centered Y1H protocol could be an optimal choice. In this case, a cis-element library is screened against TF bait, so that it could efficiently help eliminate false positives caused by endogenous TFs’ binding on the bait sequence [Citation 37, Citation 38]. It is ... fish in hawaii picturesWeb7 Jun 2024 · The TF-centered Y1H (protein-to-DNA) method based on the Y1H system (Ji et al. 2014a) allowed us to screen for new motifs bound by BplMYB46. This system does not require a specific antibody against the protein of interest, nor does it use genomic methods, such as microarrays or high-throughput sequencing or removal of non-binding sequences … can chemotherapy affect your heart